abstract：:This work aims at investigating the effect of the nanoemulsion delivery systems on the antimicrobial activity of different essential oil components. Carvacrol, limonene and cinnamaldehyde were encapsulated in the sunflower oil droplets of nanoemulsions prepared by high pressure homogenization and stabilized by differe...

journal_title：Journal of biotechnology

authors： Donsì F,Annunziata M,Vincensi M,Ferrari G

更新日期：2012-06-30 00:00:00

abstract：:Candida antarctica lipase B (CALB) has been employed as an efficient catalyst in the preparation of many flavor esters. A CALB-displaying yeast whole-cell biocatalyst could be an attractive alternative to commercial immobilized CALB because of its low-cost preparation and high enzymatic activity. We investigated the p...

journal_title：Journal of biotechnology

authors： Jin Z,Ntwali J,Han SY,Zheng SP,Lin Y

更新日期：2012-05-31 00:00:00

abstract：:Many representatives of the order Actinomycetales are prolific producers of thousands of biologically active secondary metabolites. Actinomycetes from terrestrial sources have been studied and screened since the 1950s, yielding many important anti-infective and anti-cancer drugs. However, frequent re-discovery of the ...

journal_title：Journal of biotechnology

authors： Zotchev SB

更新日期：2012-04-30 00:00:00

abstract：:Corynebacterium glutamicum possesses export systems for various amino acids including BrnFE, a two-component export system for L-methionine and the branched-chain amino acids L-valine, L-isoleucine and L-leucine. A gene for a putative transcriptional regulator of the Lrp family is transcribed divergently to the brnFE ...

journal_title：Journal of biotechnology

authors： Lange C,Mustafi N,Frunzke J,Kennerknecht N,Wessel M,Bott M,Wendisch VF

更新日期：2012-04-30 00:00:00

abstract：:Structural composition and gene content of a biogas-producing microbial community from a production-scale biogas plant fed with renewable primary products was recently analyzed by means of a metagenome sequencing approach. To determine the transcriptionally active part of the same biogas community and to identify key ...

journal_title：Journal of biotechnology

authors： Zakrzewski M,Goesmann A,Jaenicke S,Jünemann S,Eikmeyer F,Szczepanowski R,Al-Soud WA,Sørensen S,Pühler A,Schlüter A

更新日期：2012-04-30 00:00:00

abstract：:Oxygen tension regulates the biological characteristics of hematopoietic stem and progenitor cells (HSPCs) by modulating intracellular reactive oxygen species (ROS). To better understand oxygen tension mechanism on HSPCs culture, gene expression analysis of human CD34(+)CD38(-) HSPCs was performed using microarrays. T...

journal_title：Journal of biotechnology

authors： Fan J,Cai H,Li Q,Du Z,Tan W

更新日期：2012-04-15 00:00:00

abstract：:Skeletal muscle is becoming an attractive target tissue for gene therapy. Nevertheless, the low level of gene therapeutic expression in this tissue is the major limitation to it becoming an ideal target for gene transfer. The promoter is important element for gene transcription; however, the gene expression efficienci...

journal_title：Journal of biotechnology

authors： Jianwei D,Qianqian Z,Songcai L,Mingjun Z,Xiaohui R,Linlin H,Qingyan J,Yongliang Z

更新日期：2012-04-15 00:00:00

abstract：:The large scale production of a monoclonal anti-progesterone antibody in serum free medium followed by affinity chromatography on protein G lead to a contamination of the antibody sample with a protein of about 14 kDa. This protein was identified by mass spectrometry as secretory leukocyte protease inhibitor (SLPI). T...

journal_title：Journal of biotechnology

authors： Schenk JA,Fettke J,Lenz C,Albers K,Mallwitz F,Gajovic-Eichelmann N,Ehrentreich-Förster E,Kusch E,Sellrie F

更新日期：2012-03-31 00:00:00

abstract：:In the present investigation, a detailed biochemical analysis of the high H₂ producer D1 protein mutant strain L159I-N230Y of Chlamydomonas reinhardtii, carrying a double amino acid substitution, was made. The leucine residue L159 was replaced by isoleucine, and the N230 asparagine was replaced by tyrosine. The perfor...

journal_title：Journal of biotechnology

authors： Scoma A,Krawietz D,Faraloni C,Giannelli L,Happe T,Torzillo G

更新日期：2012-02-20 00:00:00

abstract：:Harbour sediments are periodically subjected to dredging operations and their management is mainly based on the assessment of the chemical contamination levels, but the potential risks posed by the presence of pathogenic microorganisms have been largely neglected. Here we first developed new molecular protocols based ...

journal_title：Journal of biotechnology

authors： Luna GM,Dell'Anno A,Pietrangeli B,Danovaro R

更新日期：2012-02-20 00:00:00

abstract：:To study the influence of N-linked carbohydrate moiety on the catalytic and biochemical properties of glycosylated enzyme, a recombinant β-d-glucuronidase (PGUS-P) from Penicillium purpurogenum as a model glycoprotein, was deglycosylated with peptide-N-glycosidase F (PNGase-F) under native conditions. The enzymatic de...

journal_title：Journal of biotechnology

authors： Zou S,Xie L,Liu Y,Kaleem I,Zhang G,Li C

更新日期：2012-02-10 00:00:00

abstract：:Microbial host-pathogen interactions have been traditionally well studied at genetic and physiological levels, but cell-resolution analyses have been particularly scarce. This has been especially remarkable for intracellular parasites for two major reasons: first, the inherent loss of bacteria traceability once infect...

journal_title：Journal of biotechnology

authors： Medina C,Santero E,Gómez-Skarmeta JL,Royo JL

更新日期：2012-02-10 00:00:00

abstract：:Poly-l-cysteine (PLCys) is drawing attention as a potential sorbent of thiol (SH)-reactive toxic heavy metal ions in the wastewater and polluted soils. However, preparation of PLCys relies on chemically synthesized polymers, in which SH groups must be protected and deprotected prior to use. On the other hand, α-chymot...

journal_title：Journal of biotechnology

authors： Narai-Kanayama A,Hanaishi T,Aso K

更新日期：2012-02-10 00:00:00

abstract：:Microbial communities are under constant influence of physical and chemical components in ecosystems. Shifts in conditions such as pH, temperature or carbon source concentration can translate into shifts in overall ecosystem functioning. These conditions can be manipulated in a laboratory setup using evolutionary comp...

journal_title：Journal of biotechnology

authors： Kucharzyk KH,Crawford RL,Paszczynski AJ,Soule T,Hess TF

更新日期：2012-01-01 00:00:00

abstract：:In this study, we developed a simple label-free method for the detection of catalase (CAT) using liquid crystals (LCs). The optical appearance of LCs changed from bright to dark when hydrogen peroxide was in contact with the dodecanal-doped nematic LC, 4-cyano-4'-pentylbiphenyl (5CB). Since hydrogen peroxide can oxidi...

journal_title：Journal of biotechnology

authors： Hu QZ,Jang CH

更新日期：2012-01-01 00:00:00

abstract：:Each of the twelve enzymes for glycolytic fermentation, eleven from Escherichia coli and one from Saccharomyces cerevisiae, have been over-expressed in E. coli and purified with His-tags. Simple assays have been developed for each enzyme and they have been assembled for fermentation of glucose to ethanol. Phosphorus-3...

journal_title：Journal of biotechnology

authors： Stevenson BJ,Liu JW,Kuchel PW,Ollis DL

更新日期：2012-01-01 00:00:00

abstract：:The production of the blue pigment indigoidine has been achieved in the entomopathogenic bacterium Photorhabdus luminescens by a promoter exchange and in Escherichia coli following heterologous expression of the biosynthesis gene indC. Moreover, genes involved in the regulation of this previously "silent" biosynthesis...

journal_title：Journal of biotechnology

authors： Brachmann AO,Kirchner F,Kegler C,Kinski SC,Schmitt I,Bode HB

更新日期：2012-01-01 00:00:00

abstract：:Old yellow enzymes (OYEs, EC 1.6.99.1) are flavin-dependent oxidoreductases that catalyze the stereoselective trans-hydrogenation of the double bond, representing a promising alternative to metal-based catalysis. Bioconversion of ketoisophorone (KIP) by 28 non-conventional yeasts belonging to 16 different species was ...

journal_title：Journal of biotechnology

authors： Raimondi S,Romano D,Amaretti A,Molinari F,Rossi M

更新日期：2011-12-20 00:00:00

abstract：:Nanoliposomes containing phosphatidic acid or cardiolipin are able to target in vitro with very high affinity amyloid-β (Aβ), a peptide whose overproduction and progressive aggregation in the brain play a central role in the pathogenesis of Alzheimer's disease. However, the presence of the blood-brain barrier (BBB) se...

journal_title：Journal of biotechnology

authors： Re F,Cambianica I,Sesana S,Salvati E,Cagnotto A,Salmona M,Couraud PO,Moghimi SM,Masserini M,Sancini G

更新日期：2011-12-20 00:00:00

abstract：:In the two-step fermentative production of vitamin C, its precursor 2-keto-L-gulonic acid (2-KLG) was synthesized by Ketogulonicigenium vulgare through co-culture with Bacillus megaterium. The reconstruction of the amino acid metabolic pathway through completed genome sequence annotation demonstrated that K. vulgare w...

journal_title：Journal of biotechnology

authors： Liu L,Chen K,Zhang J,Liu J,Chen J

更新日期：2011-12-10 00:00:00

abstract：:Glycolate(GL)-based polyesters were for the first time produced in the recombinant Escherichia coli fatty acid β-oxidation pathway reinforcing mutant LS5218, using extracellularly added GL as a monomer precursor. Cells expressing a Ser325Thr/Gln481Lys mutant of polyhydroxyalkanoate synthase (PhaC1STQK) from Pseudomona...

journal_title：Journal of biotechnology

authors： Matsumoto K,Ishiyama A,Sakai K,Shiba T,Taguchi S

更新日期：2011-12-10 00:00:00

abstract：:A new β-glucosidase gene (bglSp) was cloned from the ginsenoside converting Sphingomonas sp. strain 2F2 isolated from the ginseng cultivating filed. The bglSp consisted of 1344 bp (447 amino acid residues) with a predicted molecular mass of 49,399 Da. A BLAST search using the bglSp sequence revealed significant homolo...

journal_title：Journal of biotechnology

authors： Wang L,Liu QM,Sung BH,An DS,Lee HG,Kim SG,Kim SC,Lee ST,Im WT

更新日期：2011-11-10 00:00:00

abstract：:Verapamil has been shown to inhibit glucose transport in several cell types. However, the consequences of this inhibition on central metabolism are not well known. In this study we focused on verapamil induced changes in metabolic fluxes in a murine atrial cell line (HL-1 cells). These cells were adapted to serum free...

journal_title：Journal of biotechnology

authors： Strigun A,Noor F,Pironti A,Niklas J,Yang TH,Heinzle E

更新日期：2011-09-20 00:00:00

abstract：:MicroRNAs (miRNAs) are gaining recognition as essential regulators involved in many biological processes, and they are emerging as therapeutic targets for treating disease. Here, we introduce a method for effective delivery of anti-miRNA oligonucleotides (AMOs) using functionalized gold nanoparticles (AuNPs). To demon...

journal_title：Journal of biotechnology

authors： Kim JH,Yeom JH,Ko JJ,Han MS,Lee K,Na SY,Bae J

更新日期：2011-09-20 00:00:00

abstract：:Agrobacterium sp. H13-3, formerly known as Rhizobium lupini H13-3, is a soil bacterium that was isolated from the rhizosphere of Lupinus luteus. The isolate has been established as a model system for studying novel features of flagellum structure, motility and chemotaxis within the family Rhizobiaceae. The complete ge...

journal_title：Journal of biotechnology

authors： Wibberg D,Blom J,Jaenicke S,Kollin F,Rupp O,Scharf B,Schneiker-Bekel S,Sczcepanowski R,Goesmann A,Setubal JC,Schmitt R,Pühler A,Schlüter A

更新日期：2011-08-20 00:00:00

abstract：:As the understanding of variation is the key to a good process and product quality one should pay attention to dynamics on the single-cell level. The basic idea of this approach was to qualify and quantify variations on the single-cell level during bioreactor cultivations by monitoring the expression of an eGFP tagged...

journal_title：Journal of biotechnology

authors： Broger T,Odermatt RP,Huber P,Sonnleitner B

更新日期：2011-07-20 00:00:00

abstract：:The efficient separation of Corynebacterium glutamicum cells from culture medium by size exclusion chromatography (SEC) is presented. Residue analysis demonstrated that this method effectively depletes extracellular compounds. For evaluation, SEC was compared with the common methods cold methanol treatment, fast centr...

journal_title：Journal of biotechnology

authors： Persicke M,Plassmeier J,Neuweger H,Rückert C,Pühler A,Kalinowski J

更新日期：2011-07-10 00:00:00

abstract：:Corynebacterium glutamicum produces succinate from glucose via the reductive tricarboxylic acid cycle under microaerobic and anaerobic conditions. We identified a NCgl2130 gene of C. glutamicum as a novel succinate exporter that functions in succinate production, and designated sucE1. sucE1 expression levels were high...

journal_title：Journal of biotechnology

authors： Fukui K,Koseki C,Yamamoto Y,Nakamura J,Sasahara A,Yuji R,Hashiguchi K,Usuda Y,Matsui K,Kojima H,Abe K

更新日期：2011-06-10 00:00:00

abstract：:To overcome the low efficiency of agronomic protection from maize dwarf mosaic disease, susceptible maize inbred line was transformed by Agrobacterium tumefaciens harboring hpRNA expression vectors containing inverted-repeat sequences of different lengths targeting coat protein gene (CP) of maize dwarf mosaic virus (M...

journal_title：Journal of biotechnology

authors： Zhang ZY,Yang L,Zhou SF,Wang HG,Li WC,Fu FL

更新日期：2011-05-20 00:00:00

abstract：:Two-stage fermentation process was used for lipid production by Lipomyces starkeyi AS 2.1560 in glucose solution without auxiliary nutrients. In the first stage, cells were cultivated in a nutrient-rich medium for propagation. In the second stage, cells were resuspended in glucose solution to achieve high cellular lip...

journal_title：Journal of biotechnology

authors： Lin J,Shen H,Tan H,Zhao X,Wu S,Hu C,Zhao ZK

更新日期：2011-04-10 00:00:00

abstract：:Streptavidin is tetrameric protein which has tight and specific biotin binding affinity, and streptavidin modification of proteins or small molecules is widely used for biotechnology tool. Here, we demonstrate site-specific streptavidin-protein conjugation using enzymes. We focused on sortase A, a transpeptidase from ...

journal_title：Journal of biotechnology

authors： Matsumoto T,Sawamoto S,Sakamoto T,Tanaka T,Fukuda H,Kondo A

更新日期：2011-03-10 00:00:00

abstract：:RNF2 is a core component of Polycomb repressive complex 1 (PRC1), which binds to various chromatic regions to regulate their transcriptional activity. It was recently reported that EZH2, a Polycomb group complex member, helps mediate long-range chromosomal interactions in mammalian cells. The present study investigate...

journal_title：Journal of biotechnology

authors： Choi D,Goo HG,Yoo J,Kang S

更新日期：2011-02-20 00:00:00

abstract：:Artificial microRNA (amiRNA) technology has been applied in Arabidopsis thaliana and other plants to efficiently silence target genes of interest. Here we described a novel approach to construct plant amiRNA expression vectors with seamless enzyme-free cloning (SEFC) and mating-assisted genetically integrated cloning ...

journal_title：Journal of biotechnology

authors： Yan H,Deng X,Cao Y,Huang J,Ma L,Zhao B

更新日期：2011-01-10 00:00:00

abstract：:RNA molecules play vital informational, structural, and functional roles in molecular biology, making them ideal targets for synthetic biology. However, several challenges remain for engineering novel allosteric RNA molecules, and the development of efficient computational design techniques is vitally needed. Here we ...

journal_title：Journal of biotechnology

authors： Shu W,Liu M,Chen H,Bo X,Wang S

更新日期：2010-12-01 00:00:00

abstract：:Escherichia coli secreted protein A (EspA) is a component of the type 3 secretion system (T3SS). The high level of expression when self-stimulated suggests that EspA may be used as a fusion partner. In the present study, EspA was used as a "fusion partner" to construct a fusion expression system, pEspA, in order to im...

journal_title：Journal of biotechnology

authors： Cheng Y,Gu J,Wang HG,Yu S,Liu YQ,Ning YL,Zou QM,Yu XJ,Mao XH

更新日期：2010-11-01 00:00:00

abstract：:Papain-catalyzed polymerization of L-tyrosine ethyl ester in aqueous media was efficient for synthesis of oligo-tyrosine peptides having angiotensin I-converting enzyme inhibitory activity. Di-, tri-, and tetra-tyrosine accumulated in the soluble fraction of reaction mixture. On the other hand, the peptide products wi...

journal_title：Journal of biotechnology

authors： Narai-Kanayama A,Shikata Y,Hosono M,Aso K

更新日期：2010-11-01 00:00:00

abstract：:"CloneAssistant 1.0" is a stand-alone software compatible with the current Windows operating systems, which can automatically design cloning primers with full consideration of the sequence information of vectors and genes, cloning strategies, the principles of primer design, reading frames, position effects, and enzym...

journal_title：Journal of biotechnology

authors： Shao C,Meng Y,Lv S,Zhong W,Wang Z,Chen M

更新日期：2010-11-01 00:00:00

abstract：:Xylose-fermenting Saccharomyces strains are needed for commercialization of ethanol production from lignocellulosic biomass. Engineered Saccharomyces cerevisiae strains expressing XYL1, XYL2 and XYL3 from Pichia stipitis, however, utilize xylose in an oxidative manner, which results in significantly lower ethanol yiel...

journal_title：Journal of biotechnology

authors： Kim SR,Lee KS,Choi JH,Ha SJ,Kweon DH,Seo JH,Jin YS

更新日期：2010-11-01 00:00:00

abstract：:While phase I and phase II drug metabolites are important for drug development and toxicity studies, e.g. in the context of metabolites in safety testing (MIST), they are often not commercially available and their classical chemical synthesis can be cumbersome. Therefore, a biotechnological production of drug metaboli...

journal_title：Journal of biotechnology

authors： Naumann JM,Messinger J,Bureik M

更新日期：2010-10-01 00:00:00

abstract：:The development of SIN-lentivectors has paved the way for the double copy vectors (DCV), which substitute the deletion in the 3'LTR with either transgenic or insulator sequences. However, the limits of this approach remain unclear. Previous results have demonstrated that transduction efficiencies of DCV carrying large...

journal_title：Journal of biotechnology

authors： Bos TJ,De Bruyne E,Van Lint S,Heirman C,Vanderkerken K

更新日期：2010-10-01 00:00:00